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microscope stage warmers  (Tokai Hit Co Ltd)

 
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    Tokai Hit Co Ltd microscope stage warmers
    Microscope Stage Warmers, supplied by Tokai Hit Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microscope+stage+warmers/stage+heater/us07972849-820-10-13
    Average 90 stars, based on 1 article reviews
    microscope stage warmers - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Micromanipulation:

    Article Title: Epigenetic Reprogramming by Somatic Cell Nuclear Transfer in Primates
    Article Snippet: .. All nuclear transfer micromanipulation and fusion procedures were conducted on microscope stage warmers (Tokai Hit) maintaining 37°C. .. Reconstructed oocytes were activated 2 hours post-fusion by a 5-minute exposure to 5 μ M ionomycin (CalBiochem, San Diego, http://www.emdbiosciences.com ) followed by a 5-hour incubation in 2 mM 6-dimethylaminopurine.

    Article Title: Primate pluripotent stem cells produced by somatic cell nuclear transfer
    Article Snippet: .. All nuclear transfer micromanipulation and fusion procedures were conducted on microscope stage warmers (Tokai Hit) maintaining 37° C. Reconstructed embryos were activated by exposure to 5 μM ionomycin for 5 min in TALP/HEPES medium supplemented with 1 mg/ml fatty acid-free bovine serum albumin (BSA) and then transferred for 5 min in TALP/HEPES medium supplemented with 30 mg/ml fatty acid-free BSA and 2 mM 6-dimethylaminopurine (DMAP) followed by a 5 hour incubation in HECM-9 medium containing 2 mM DMAP at 37° C. in 6% CO2. ..

    Article Title: Producing primate embryonic stem cells by somatic cell nuclear transfer.
    Article Snippet: Derivation of embryonic stem (ES) cells genetically identical to a patient by somatic cell nuclear transfer (SCNT) holds the potential to cure or alleviate the symptoms of many degenerative diseases while circumventing concerns regarding rejection by the host immune system.. However, the concept has only been achieved in the mouse, whereas inefficient reprogramming and poor embryonic development characterizes the results obtained in primates.. Here, we used a modified SCNT approach to produce rhesus macaque blastocysts from adult skin fibroblasts, and successfully isolated two ES cell lines from these embryos.

    Microscopy:

    Article Title: Epigenetic Reprogramming by Somatic Cell Nuclear Transfer in Primates
    Article Snippet: .. All nuclear transfer micromanipulation and fusion procedures were conducted on microscope stage warmers (Tokai Hit) maintaining 37°C. .. Reconstructed oocytes were activated 2 hours post-fusion by a 5-minute exposure to 5 μ M ionomycin (CalBiochem, San Diego, http://www.emdbiosciences.com ) followed by a 5-hour incubation in 2 mM 6-dimethylaminopurine.

    Article Title: Primate pluripotent stem cells produced by somatic cell nuclear transfer
    Article Snippet: .. All nuclear transfer micromanipulation and fusion procedures were conducted on microscope stage warmers (Tokai Hit) maintaining 37° C. Reconstructed embryos were activated by exposure to 5 μM ionomycin for 5 min in TALP/HEPES medium supplemented with 1 mg/ml fatty acid-free bovine serum albumin (BSA) and then transferred for 5 min in TALP/HEPES medium supplemented with 30 mg/ml fatty acid-free BSA and 2 mM 6-dimethylaminopurine (DMAP) followed by a 5 hour incubation in HECM-9 medium containing 2 mM DMAP at 37° C. in 6% CO2. ..

    Article Title: Producing primate embryonic stem cells by somatic cell nuclear transfer.
    Article Snippet: Derivation of embryonic stem (ES) cells genetically identical to a patient by somatic cell nuclear transfer (SCNT) holds the potential to cure or alleviate the symptoms of many degenerative diseases while circumventing concerns regarding rejection by the host immune system.. However, the concept has only been achieved in the mouse, whereas inefficient reprogramming and poor embryonic development characterizes the results obtained in primates.. Here, we used a modified SCNT approach to produce rhesus macaque blastocysts from adult skin fibroblasts, and successfully isolated two ES cell lines from these embryos.

    Incubation:

    Article Title: Primate pluripotent stem cells produced by somatic cell nuclear transfer
    Article Snippet: .. All nuclear transfer micromanipulation and fusion procedures were conducted on microscope stage warmers (Tokai Hit) maintaining 37° C. Reconstructed embryos were activated by exposure to 5 μM ionomycin for 5 min in TALP/HEPES medium supplemented with 1 mg/ml fatty acid-free bovine serum albumin (BSA) and then transferred for 5 min in TALP/HEPES medium supplemented with 30 mg/ml fatty acid-free BSA and 2 mM 6-dimethylaminopurine (DMAP) followed by a 5 hour incubation in HECM-9 medium containing 2 mM DMAP at 37° C. in 6% CO2. ..



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    Chromium (Cr) (VI) damaged male reproductive system and spermatogonial stem cells (SSCs)/progenitors in mouse testes. (A) Hematoxylin and eosin (H&E) staining of testis sections from control and Cr (VI)-treated mice. Bar = 200 μm. (B–D) The ratios of only 1–3 layers of germ cell (B) , empty (C) , and abnormal (D) seminiferous tubules in control and Cr (VI)-treated mouse testes. (E) The average body weight in control and Cr (VI)-treated mice. (F) The average testicular index (testis weight/body weight) in control and Cr (VI)-treated mice. (G) The average sperm number (10 6 /ml) in control and Cr (VI)-treated mice. (H) The ratio of <t>spermatozoa</t> with progressive motility in control and Cr (VI)-treated mice. (I) Left: the ratio of abnormal spermatozoa in control and Cr (VI)-treated mice; Right: images of spermatozoa from the control and Cr (VI)-treated mice. Bar = 50 μm. (J,K) Immunofluorescence staining for LIN28 (J) and CDH1 (K) in testis sections from control and Cr (VI)-treated mice. Bar = 100 μm. (L,M) The numbers of LIN28 + (L) and CDH1 + cells (M) per seminiferous tubule in control and Cr (VI)-treated mouse testes. Data are presented as the mean ± SEM from five mice, and 300 seminiferous tubules from five mice were analyzed in each group. ** p < 0.01.
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    Chromium (Cr) (VI) damaged male reproductive system and spermatogonial stem cells (SSCs)/progenitors in mouse testes. (A) Hematoxylin and eosin (H&E) staining of testis sections from control and Cr (VI)-treated mice. Bar = 200 μm. (B–D) The ratios of only 1–3 layers of germ cell (B) , empty (C) , and abnormal (D) seminiferous tubules in control and Cr (VI)-treated mouse testes. (E) The average body weight in control and Cr (VI)-treated mice. (F) The average testicular index (testis weight/body weight) in control and Cr (VI)-treated mice. (G) The average sperm number (10 6 /ml) in control and Cr (VI)-treated mice. (H) The ratio of <t>spermatozoa</t> with progressive motility in control and Cr (VI)-treated mice. (I) Left: the ratio of abnormal spermatozoa in control and Cr (VI)-treated mice; Right: images of spermatozoa from the control and Cr (VI)-treated mice. Bar = 50 μm. (J,K) Immunofluorescence staining for LIN28 (J) and CDH1 (K) in testis sections from control and Cr (VI)-treated mice. Bar = 100 μm. (L,M) The numbers of LIN28 + (L) and CDH1 + cells (M) per seminiferous tubule in control and Cr (VI)-treated mouse testes. Data are presented as the mean ± SEM from five mice, and 300 seminiferous tubules from five mice were analyzed in each group. ** p < 0.01.
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    Image Search Results


    Chromium (Cr) (VI) damaged male reproductive system and spermatogonial stem cells (SSCs)/progenitors in mouse testes. (A) Hematoxylin and eosin (H&E) staining of testis sections from control and Cr (VI)-treated mice. Bar = 200 μm. (B–D) The ratios of only 1–3 layers of germ cell (B) , empty (C) , and abnormal (D) seminiferous tubules in control and Cr (VI)-treated mouse testes. (E) The average body weight in control and Cr (VI)-treated mice. (F) The average testicular index (testis weight/body weight) in control and Cr (VI)-treated mice. (G) The average sperm number (10 6 /ml) in control and Cr (VI)-treated mice. (H) The ratio of spermatozoa with progressive motility in control and Cr (VI)-treated mice. (I) Left: the ratio of abnormal spermatozoa in control and Cr (VI)-treated mice; Right: images of spermatozoa from the control and Cr (VI)-treated mice. Bar = 50 μm. (J,K) Immunofluorescence staining for LIN28 (J) and CDH1 (K) in testis sections from control and Cr (VI)-treated mice. Bar = 100 μm. (L,M) The numbers of LIN28 + (L) and CDH1 + cells (M) per seminiferous tubule in control and Cr (VI)-treated mouse testes. Data are presented as the mean ± SEM from five mice, and 300 seminiferous tubules from five mice were analyzed in each group. ** p < 0.01.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Melatonin Attenuates Chromium (VI)-Induced Spermatogonial Stem Cell/Progenitor Mitophagy by Restoration of METTL3-Mediated RNA N 6 -Methyladenosine Modification

    doi: 10.3389/fcell.2021.684398

    Figure Lengend Snippet: Chromium (Cr) (VI) damaged male reproductive system and spermatogonial stem cells (SSCs)/progenitors in mouse testes. (A) Hematoxylin and eosin (H&E) staining of testis sections from control and Cr (VI)-treated mice. Bar = 200 μm. (B–D) The ratios of only 1–3 layers of germ cell (B) , empty (C) , and abnormal (D) seminiferous tubules in control and Cr (VI)-treated mouse testes. (E) The average body weight in control and Cr (VI)-treated mice. (F) The average testicular index (testis weight/body weight) in control and Cr (VI)-treated mice. (G) The average sperm number (10 6 /ml) in control and Cr (VI)-treated mice. (H) The ratio of spermatozoa with progressive motility in control and Cr (VI)-treated mice. (I) Left: the ratio of abnormal spermatozoa in control and Cr (VI)-treated mice; Right: images of spermatozoa from the control and Cr (VI)-treated mice. Bar = 50 μm. (J,K) Immunofluorescence staining for LIN28 (J) and CDH1 (K) in testis sections from control and Cr (VI)-treated mice. Bar = 100 μm. (L,M) The numbers of LIN28 + (L) and CDH1 + cells (M) per seminiferous tubule in control and Cr (VI)-treated mouse testes. Data are presented as the mean ± SEM from five mice, and 300 seminiferous tubules from five mice were analyzed in each group. ** p < 0.01.

    Article Snippet: A minimum of 300 spermatozoa were observed from at least five randomly selected fields with 20 μm CELL-VU® DRM-600 sperm count slides (Millennium Sciences, United States) and a microscopic stage warmer (KITAZATO, Japan).

    Techniques: Staining, Control, Immunofluorescence

    Melatonin alleviated Cr (VI)-induced damage to male reproductive system and autophagy in mouse testes. (A) H&E staining of testis sections from the control, Cr (VI), melatonin + Cr (VI), and melatonin-treated mice. Bar = 200 μm. (B–D) The ratios of only 1–3 layers of germ cell (B) , empty (C) , and abnormal (D) seminiferous tubules in testes from the control, Cr (VI), melatonin + Cr (VI), and melatonin-treated mice. (E) The average body weight in different mouse groups. (F) The average testicular index (testis weight/body weight) in different mouse groups. (G) The average sperm number (10 6 /ml) in different mouse groups. (H) The ratio of spermatozoa with progressive motility in different mouse groups. (I) The upper panel: the ratio of abnormal spermatozoa in different mouse groups; The lower panel: images of spermatozoa from different groups of mice. Bar = 50 μm. (J) Immunofluorescence staining for LIN28 in testis sections from the control, Cr (VI), melatonin + Cr (VI), and melatonin-treated mice. Bar = 200 μm. (K) The numbers of LIN28 + cells per seminiferous tubule in testes from the control, Cr (VI), melatonin + Cr (VI), and melatonin-treated mice. (L) Co-staining analysis for LC3 and LIN28 in testis sections from the control, Cr (VI), melatonin + Cr (VI), and melatonin-treated mice. Bar = 100 μm. Data are presented as the mean ± SEM from five mice, and 300 seminiferous tubules from five mice were analyzed in each group. * p < 0.05; ** p < 0.01.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Melatonin Attenuates Chromium (VI)-Induced Spermatogonial Stem Cell/Progenitor Mitophagy by Restoration of METTL3-Mediated RNA N 6 -Methyladenosine Modification

    doi: 10.3389/fcell.2021.684398

    Figure Lengend Snippet: Melatonin alleviated Cr (VI)-induced damage to male reproductive system and autophagy in mouse testes. (A) H&E staining of testis sections from the control, Cr (VI), melatonin + Cr (VI), and melatonin-treated mice. Bar = 200 μm. (B–D) The ratios of only 1–3 layers of germ cell (B) , empty (C) , and abnormal (D) seminiferous tubules in testes from the control, Cr (VI), melatonin + Cr (VI), and melatonin-treated mice. (E) The average body weight in different mouse groups. (F) The average testicular index (testis weight/body weight) in different mouse groups. (G) The average sperm number (10 6 /ml) in different mouse groups. (H) The ratio of spermatozoa with progressive motility in different mouse groups. (I) The upper panel: the ratio of abnormal spermatozoa in different mouse groups; The lower panel: images of spermatozoa from different groups of mice. Bar = 50 μm. (J) Immunofluorescence staining for LIN28 in testis sections from the control, Cr (VI), melatonin + Cr (VI), and melatonin-treated mice. Bar = 200 μm. (K) The numbers of LIN28 + cells per seminiferous tubule in testes from the control, Cr (VI), melatonin + Cr (VI), and melatonin-treated mice. (L) Co-staining analysis for LC3 and LIN28 in testis sections from the control, Cr (VI), melatonin + Cr (VI), and melatonin-treated mice. Bar = 100 μm. Data are presented as the mean ± SEM from five mice, and 300 seminiferous tubules from five mice were analyzed in each group. * p < 0.05; ** p < 0.01.

    Article Snippet: A minimum of 300 spermatozoa were observed from at least five randomly selected fields with 20 μm CELL-VU® DRM-600 sperm count slides (Millennium Sciences, United States) and a microscopic stage warmer (KITAZATO, Japan).

    Techniques: Staining, Control, Immunofluorescence